1. It's now or never.机不可失，时不再来

Much as I hate to go, it's now or never.

虽然我很讨厌去，但也就此一回了。

2. Seize 抓住；把握

This was a once-in-a-lifetime opportunity. You must seize it.

这是千载难逢的一次机会,你一定要抓住它。

seize the day

把握今天，及时行乐

3. Grasp this opportunity 抓住机会

We will grasp this opportunity and do our best.

我们肯定会捉住这次机会并为之竭尽全力。

4. Slip through one's fingers 从指间溜走

Don't let this opportunity slip through your fingers.

千万别让这个机会溜走。

5. Take the chance 试一试，碰碰运气；抓住机会

Just take the chance.

试一试。