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杂质方法学研究 英文53

发布者: lorespirit | 发布时间: 2012-8-26 16:58| 查看数: 727| 评论数: 0|

Buffer. Ensure that the buffer (pK a ) is appropriate for the pH of the solution.

In general, to provide appropriate buffering capacity, the pH of the solution

should be within ± 1 pH unit of the pK a value of the buffer.

žIsocratic method. Whenever possible, use isocratic HPLC condition, as this

is affected less by the variation in flow rate, temperature, and dwell vol-

ume. If gradient HPLC conditions have to be used, a simple linear gradient

is preferred over multistep gradients. Complicated gradient conditions are

more susceptible to differences between HPLC instruments (e.g., flow rate,

dwell volume).

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